Figure 3

Reduced numbers of memory and memory-precursor CD8⁺ T cells in CD4-Cre A20^fl/fl mice.

CD4-Cre A20^fl/fl and A20^fl/fl control mice were infected with a non-lethal dose of Lm OVA and spleens were analyzed for T_mem formation at the indicated time points. (a) Kinetic of Lm OVA-specific CD8⁺ T cells, stained with H2-Kb SIINFEKL pentamer and gated on singlets and CD3⁺ CD8⁺ cells. (b) Absolute numbers of total (living, apoptotic and necroptotic) Lm OVA-specific CD8⁺ T cells. (c) Relative numbers of living (annexin⁻/7-AAD⁻) Lm OVA-specific CD8⁺ T cells. (d) Absolute numbers of living (annexin⁻/7-AAD⁻) Lm OVA-specific CD8⁺ T cells. (e) Representative dot plots with frequencies of Lm OVA-specific CD8⁺ SLEC (KLRG-1⁺ CD127⁻) and MPEC (KLRG-1⁻ CD127⁺) were determined. (f) Absolute numbers of Lm OVA-specific CD8⁺ SLEC. (g) Absolute numbers of Lm OVA-specific CD8⁺ MPEC. (h) Representative dot plots with frequencies of T_eff (CD62L^low and CD127^low), T_EM (CD62L^low and CD127^high) and T_CM (CD62L^high and CD127^high) were determined at day 50 and 53 p.i. (i) Absolute numbers of Lm OVA-specific CD8⁺ T_eff, T_EM and T_CM cells. Data are compiled of 2 independent experiments with 3–5 animals per group and experiment. Error bars indicate + SEM. Student’s t-test, *p < 0.05; **p < 0.01.