Figure 6: hGcmb^R47L induces Repo-positive cells similarly as hGcmb.

(a) Protein extracts from S2 cells transfected with gcm or hgcmb were harvested and analyzed in the presence or absence of CHX. Note the persistent protein levels up to 14 hours for hGcmb. Western blot gels have been run under the same experimental conditions. (b–d) Confocal projections of Stage 15 embryos expressing hgcmb and hgcmb^R47L were labeled with HRP (Magenta) and Repo (Green) for the following genotypes: Sca-Gal4 > w¹¹¹⁸ (b–b”, control), Sca-Gal4 > hgcmb (c–c”), and Sca-Gal4 > hgcmb^R47L (d–d”). Scale bar: 100 μm. (e) Statistics for the glial cell number per hemisegment were shown for embryos carrying the above genotypes. n = 40 (w¹¹¹⁸ control), n = 51 (hgcmb), and n = 40 (hgcmb^R47L). Note a significant difference between embryos expressing hgcmb or hgcmb^R47L and the control. Averages are mean ± SEM, *represents p < 0.05, **represents p < 0.01, and ***represents p < 0.001 by Student’s t test between two groups and one-way ANOVA test among multiple groups. ns means no significance.