Figure 3

Effect of melatonin and ABA on contents of non- enzymatic antioxidants GSH (a), AsA (b), Total glutathione (c) and ascorbate (d) in leaves of DX and GN under cold stress. The 21-day-old seedlings were pretreated with distilled water, 50 μM melatonin, 100 μM ABA, 50 μM fluridone, and 50 μM melatonin + 50 μM fluridone (MET + F) for 7 days, respectively, and then exposed to cold stress (4 °C) for 120 h. Plants treated with distilled water under the normal conditions (25 °C) served as controls. Each value represents the mean of three replicates ± SD, and different letters above the bars indicate significant differences at P < 0.05 among different treatments in the same genotype according to Duncan’s multiple range test.