Figure 1: Activation of PHF14 expression in the kidneys after pro-fibrotic insult. | Scientific Reports

Figure 1: Activation of PHF14 expression in the kidneys after pro-fibrotic insult.

From: PHF14: an innate inhibitor against the progression of renal fibrosis following folic acid-induced kidney injury

Figure 1

(A) Representative Masson and immunohistochemical staining assay images demonstrating the progression of fibrosis after folic acid administration. (B) Quantitative PCR (Q-PCR) analysis showing persistent elevation of PHF14 expression in the kidney on day 2, day 7, day 14, and day 28 after folic acid administration, compared with the sham control. (C) Western blot results showing that PHF14 protein expression increased with the progression of fibrosis. Anti-GAPDH was used to verify equivalent loading. (D–F) Semiquantitative analysis of PHF14, collagen I, and α-SMA protein abundance in the kidneys. (G) Representative immunohistochemical/immunofluorescent staining images demonstrating the elevation of PHF14 protein in fibrotic kidneys on day 28 after folic acid administration, compared with the sham control. Immunofluorescence images revealed the co-localization of elevated PHF14 and α-SMA expression in activated myofibroblasts. *P < 0.05, compared with sham controls (n = 5), Col 1, collagen I; α-SMA, α-smooth muscle actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; DAPI, 4′,6-diamidino-2-phenylindole; FA, folic acid.

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