Figure 2: TGF-β upregulates PHF14 expression in NRK-49F cells. | Scientific Reports

Figure 2: TGF-β upregulates PHF14 expression in NRK-49F cells.

From: PHF14: an innate inhibitor against the progression of renal fibrosis following folic acid-induced kidney injury

Figure 2

NRK-49F cells were treated with TGF-β (1 ng/mL) for the indicated time periods. Quantitative PCR (Q-PCR) analysis (A) and western blot results (B) demonstrating the increased expression of PHF14 protein for various periods of time. Anti-GAPDH was used to verify equivalent loading. (C) Semiquantitative analysis of protein abundances in the treated cells. (D) Immunofluorescence staining revealing the PHF14 protein expression profile in TGF-β-treated NRK-49F cells. Nuclei were visualized with DAPI (high-power field). *P < 0.05, compared with NRK-49F cells treated with TGF-β for 0 h. α-SMA, α-smooth muscle actin; TGF-β, transforming growth factor-β; p-smad3, phospho-smad3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; DAPI, 4′,6-diamidino-2-phenylindole.

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