Figure 5: PHF14 directly represses the transcription of PDGFR-α. | Scientific Reports

Figure 5: PHF14 directly represses the transcription of PDGFR-α.

From: PHF14: an innate inhibitor against the progression of renal fibrosis following folic acid-induced kidney injury

Figure 5

NRK-49F cells pretreated with scrambled or PHF14 siRNA were stimulated with TGF-β. (A) Western blot results demonstrating the increase of PDGFR-α, collagen I, and α-SMA synthesis in the PHF14-knockdown group. Anti-GAPDH was used to verify equivalent loading. (B–D) Semiquantitative analysis of PDGFR-α, collagen I, and α-SMA protein abundance in the TGF-β-treated cells. (E) The schematic diagram of the PDGFR-α promoter region and the primer design. (F) Chromatin immunoprecipitation of the PDGFR-α promoter region with anti-FLAG antibody. *P < 0.05, compared with scrambled controls (n = 3). GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PDGFR-α, platelet-derived growth factor receptor-α; NC, negative control; PC, positive control; P1–P4, primers 1 to 4; TSS, transcription start site.

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