Figure 1 | Scientific Reports

Figure 1

From: Piscidin-1-analogs with double L- and D-lysine residues exhibited different conformations in lipopolysaccharide but comparable anti-endotoxin activities

Figure 1

(A) and (B) Show the dose-dependent hemolysis of hRBCs and viability of murine 3T3 cells respectively in the presence of piscidin-1 and its analogs. (C to F) Scanning Electron Microscopy of E. coli ATCC 25922 in the absence and presence of piscidin-1 and its selected analogs; bacteria without any treatment (C), Bacteria after treatment with piscidin-1, T15,21 K piscidin-1 and T15,21dK piscidin-1 at 10 fold MIC of the respective peptide (D,E and F). (G) and (H) Show the plot of fluorescence recovery which is a measure of peptide-induced membrane permeabilization vs peptide concentration (μM) in mammalian and bacterial membrane mimetic PC/Chol (8:1, w/w) and PC/PG (3:1, w/w) lipid vesicles respectively. Symbols: Black square, piscidin-1; Red circle, T15,21K-piscidin-1; Green up triangle, T15,21dK-piscidin-1; Blue down triangle, T15K-piscidin-1 and Cyan diamond, T15dK-piscidin-1. Each data point is an average of three independent experiments and error bar represents the standard deviation.

Back to article page