Figure 3: Confocal laser scanning microscopy to look into the localization of piscidin-1 and its selected analogs onto E. coli and hRBCs. | Scientific Reports

Figure 3: Confocal laser scanning microscopy to look into the localization of piscidin-1 and its selected analogs onto E. coli and hRBCs.

From: Piscidin-1-analogs with double L- and D-lysine residues exhibited different conformations in lipopolysaccharide but comparable anti-endotoxin activities

Figure 3

(A) Study of localization of NBD-labelled piscidin-1 and its analogs namely, T15,21K-piscidin-1 and T15,21dK-piscidin-1 (both at ~5.0 μM) onto E. coli ATCC 25922 and (B) study of localization of NBD-labeled piscidin-1 and T15,21K-piscidin-1 and T15,21dK-piscidin-1 (~10.0 μM) onto hRBCs. Fluorescence and DIC images for treatment of different cells with each of the peptides are shown as marked on top of the respective panel. Panels (C) and (D) show the quantitative analysis of fluorescence intensity of E. coli and hRBCsbound to NBD-labeled piscidin-1, T15,21K-piscidin-1 and T15,21dK-piscidin-1 respectively.

Back to article page