Figure 4 | Scientific Reports

Figure 4

From: Piscidin-1-analogs with double L- and D-lysine residues exhibited different conformations in lipopolysaccharide but comparable anti-endotoxin activities

Figure 4

(A), Dose dependent LPS neutralization by the piscidin-1 and its analogs as determined by LAL assay; (B) the percentage inhibition of levels of LPS-induced secretions of TNF-α and IL-1β, respectively, in the presence of piscidin-1 and its analogs by ELISA experiments; (C) In vivo anti-LPS activity of the peptides in mice. Septic shock in BALB/c mice was induced by intraperitoneal (i.p.) injection of E. coli LPS (12 mg/kg) followed by i.p. injection of T15,21K-piscidin-1 (0.5 mg/Kg) or T15,21dK-piscidin-1 (1 mg/Kg) or polymyxin B (1 mg/Kg) ~5 min later. Mice treated with only saline was taken as control. Survival of the animals (n = 5) was monitored for 7 days (***p < 0.0001, log-rank test). (D) Percentage inhibition of TNF-α and IL-6 in LPS (12 mg/Kg) treated mice serum in the presence of piscidin-1 analogs T15,21K-piscidin-1 (0.5 mg/Kg), T15,21dK-piscidin-1 (1 mg/Kg) and polymyxin B (1 mg/Kg) by ELISA experiments.

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