Table 1 Sequence and molecular weight of piscidin-1 and its designed analogs.

From: Piscidin-1-analogs with double L- and D-lysine residues exhibited different conformations in lipopolysaccharide but comparable anti-endotoxin activities

Peptide

Sequence

Calculated mass

Observed mass

HPLC retention time

Piscidin-1

X-FFHHIFRGIVHVGKTIHRLVTG–amide

2572.38

2571.40

14.98

T15,21K Piscidin-1

X-FFHHIFRGIVHVGKKIHRLVKG–amide

2626.52

2625.30

13.40

T15,21dKpiscidin-1

X-FFHHIFRGIVHVGKkIHRLVkG–amide

2626.52

2625.30

12.38

T15K Piscidin-1

X-FFHHIFRGIVHVGKKIHRLVTG–amide

2599.45

2598.30

14.10

T15dK Piscidin-1

X-FFHHIFRGIVHVGKkIHRLVTG–amide

2599.45

2598.30

12.78

  1. *Substituted amino acids are shown as underlined.
  2. **k = D-Lysine; X = H or NBD.
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