Figure 4: Sub-acute restraint stress results in de-synchronization of the mast cell clockwork, concomitant with the absence of temporal variations in OCT3 expression and plasma histamine levels.
From: Regulation of plasma histamine levels by the mast cell clock and its modulation by stress
(A) Experimental protocol. (B) Top: Representative pictures of in vivo imaging of mast cell–deficient mice reconstituted with subcutaneous injections of BMMCs derived from PER2LUC knock-in mice, with or without Clock mutation, at the indicated time points. Bottom: A quantitative analysis of the data. (n = 6) (One-way repeated measures ANOVA). (C) OCT3 and Per2 mRNA expression at ZT4 and ZT16 in the skin of control and stressed mice. (n = 8–10) (Unpaired student’s t-test). (D) OCT3 protein expression in the peritoneal mast cells isolated from control and stressed mice at ZT4 and ZT16. (n = 8–10) (One-way ANOVA with Bonferroni’s test). (E) Plasma histamine levels at ZT4 and ZT16 in individual control and stressed mice. (n = 6–8) (Paired student’s t-test) The values represent means ± SD, *p < 0.05, **p < 0.01. Similar results (B–E) were obtained at least from 2 independent experiments.
