Figure 3: The bioactivity of FUS-facilitated viral delivery was tested via optical stimulation.
From: Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
(a) Four 2 s blue light (470 nm) pulses were given to elicit neuronal response in freely behaving mice. FUS-facilitated viral delivery was carried out in these mice (N = 3) targeting hippocampus. Sorted spike signals (top) and the corresponding number of spikes (bottom, binned into 0.4 s segments) revealed elevated neuronal activity during stimulation (red). (b) Four 2-s pulses were applied to mice (N = 3) that received direct infusion of viral vectors. An increased neuronal activity was observed during stimulation, which is similar to that of FUS-facilitated viral delivery group. (c) Examples of individual spike signals during stimulation from FUS-facilitated delivery group (red) and direct infusion group (blue). The baseline level spike for each group is indicated in black. (d) The firing rate was calculated for each group and significant increases (p < 0.001) in firing rate were observed during stimulation.
