Figure 4
From: Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
(a) Ten-second long pulses were also applied (red). An increased spike amplitude and number of spikes were observed at the onset of stimulation, which was followed by a gradual decrease to baseline level. (b) Neuronal activity (ChR2 is red) was revealed by labeling c-Fos proteins with fluorescent markers (green). (c) An example of a ChR2-expressing neuron that was activated by optical stimulation. Scale bars represent 20 μm and 5 μm in (b,c), respectively.
