Figure 1: Schematic diagram of the construction strategy for pMCMV. | Scientific Reports

Figure 1: Schematic diagram of the construction strategy for pMCMV.

From: Further characterization of Maize chlorotic mottle virus and its synergistic interaction with Sugarcane mosaic virus in maize

Figure 1

(A) Full-length MCMV genome showing positions of restriction sites. (B) PCR-amplifed digestion products that were sequentially inserted into pCB301 to produce pMCMV. Positions of duplicated 2 × 35S promoter, hepatitis delta virus antigenomic ribozyme (HDV_Rz) and NOS terminator in pCB301 are indicated. Transcriptional start site and the HDV_Rz cleavage site are indicated. (C) Organization of MCMV cDNA between the StuI and SmaI sites. Positions of individual MCMV ORFs are shown. The read-through stop codons for p50 or p7 are also indicated.

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