Figure 4: Detection of MCMV in pMCMV-inoculated maize plants. | Scientific Reports

Figure 4: Detection of MCMV in pMCMV-inoculated maize plants.

From: Further characterization of Maize chlorotic mottle virus and its synergistic interaction with Sugarcane mosaic virus in maize

Figure 4

(A) Relative accumulation of MCMV in three plants detected by ELISA and absorbance at OD405. Three plants were tested for each treatment, and each sample was tested in triplicate. (B) Western blot of MCMV using MCMV-specific monoclonal antibody. Membrane probed with a monoclonal antibody against β-actin was used as loading control. (C) Northern blot assay for MCMV RNA using a probe was specific for MCMV CP region amplified by PCR with primers MCMV/CP-F and MCMV/CP-R. Lanes 1–3, three respective pMCMV-inoculated plants. (D) TEM of negatively stained viral particles (arrow) from pMCMV inoculated plants. ck, mock-inoculated plants; pMCMV, plants inoculated with pMCMV; wt, plants rub-inoculated MCMV-YN1. Leaves were harvested at 10 dpi in (A,B,C or D).

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