Figure 5: Transmission electron micrographs of maize cells and mRNA levels for PPDK in plants infected with MCMV alone, with MCMV and SCMV, or mock-inoculated with phosphate buffer. | Scientific Reports

Figure 5: Transmission electron micrographs of maize cells and mRNA levels for PPDK in plants infected with MCMV alone, with MCMV and SCMV, or mock-inoculated with phosphate buffer.

From: Further characterization of Maize chlorotic mottle virus and its synergistic interaction with Sugarcane mosaic virus in maize

Figure 5

(A–C) Starch grains in chloroplasts in the bundle sheath cells of the mock-inoculated (A), MCMV-inoculated (B) or dual inoculation with MCMV and SCMV (C). (D) Quantitative RT-PCR to show relative PPDK expression. (E–I) Mitochondria in the mock- (E), MCMV- (F) and MCMV + SCMV-inoculated (G,H,I) cells. Disorganized cristae are marked with white arrows, and fine fibrous materials are marked with white solid arrowheads. White solid arrow in (H) indicates materials released from a disrupted mitochondria. (J) Aggregates of MCMV virions (white open arrowheads) in MCMV-infected plant cells. (K) Pinwheels (white solid arrowheads) in the SCMV infected cells. (L) Flexuous, rod-shaped virions (black open arrowheads) in the SCMV infected cells. Multi-vesicular bodies (black solid arrows) in MCMV-infected (M,N) or MCMV and SCMV co-infected cells (O,P). Spherical viral particles (black open arrowheads) were seen inside the Multi-vesicular bodies in (N). Ch, chloroplast; M, mitochondria; S, starch grains. Bars: a–c = 500 nm, e–p = 200 nm.

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