Table 3 Primers and sequences used for PCR.
Primers | Primer sequences, 5′ → 3′ | Purpose |
|---|---|---|
M1F | AGGTAATCTGCGGCAACAGACCCCAACG | For clone of MCMV cDNA |
M2R | GGGCCGGAAGAGAGGGGCATTAC | |
MCMV/CP-F | ACAGGACACCGTTGCCGTTTAT | For MCMV detection |
MCMV/CP-R | CGATTTAGGCTCCCAGACACTT | |
SCMV/F | GTGTGGAATGGTTCACTCAAAGCTG | For SCMV detection |
SCMV/R | GGTGTTGCAATTGGTGTGTACACG | |
PPDK/F | CGCCGATACAGACGACCAAAAAGAGG | For amplification of PPDK |
PPDK/R | CCCAGCAGTTCCTTCATGGTCTTGT | |
EF1α/F | TTCACACTTGGTGTGAAGCAGATG | For amplification of EF1α |
EF1α/R | TTGTATCCAACCTTCTTCAGGTAGG |
