Figure 1: Virus infection down-regulates Brd3 expression in macrophages. | Scientific Reports

Figure 1: Virus infection down-regulates Brd3 expression in macrophages.

From: Bromodomain protein Brd3 promotes Ifnb1 transcription via enhancing IRF3/p300 complex formation and recruitment to Ifnb1 promoter in macrophages

Figure 1

(a), Total RNA was extracted from different mouse tissues and immune cells, 1 μg RNA was used to perform reverse transcription-PCR. Real time-PCR was performed to analysis the Brd3 mRNA expression level, Actb was used as a control. Then the reaction product was analysed by agarose electrophoresis. (b), Mouse peritoneal macrophages were infected with HSV (MOI = 10), VSV (MOI = 10), SeV (MOI = 10) for the indicated times. The Brd3 mRNA expression level was detected by Q-PCR. The results were presented as fold expression of Brd3 mRNA to that of Actb. (c), Mouse peritoneal macrophages were treated as in b, cells were lysed and subjected to Western blot analysis(40 μg) with the indicated antibodies. Data are representative of three independent experiments with similar results (a and c), and data are shown as means ± SD of three independent experiments. *P < 0.05(analysis of variance (ANOVA)).

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