Figure 4: Brd3 promotes p300 mediated acetylation of IRF3. | Scientific Reports

Figure 4: Brd3 promotes p300 mediated acetylation of IRF3.

From: Bromodomain protein Brd3 promotes Ifnb1 transcription via enhancing IRF3/p300 complex formation and recruitment to Ifnb1 promoter in macrophages

Figure 4

(a), RAW 264.7 cells stably overexpressing TAP-tagged IRF3 were transiently transfected with Brd3-Myc plasmid or empty vector for 36 hours and then infected with VSV(MOI = 10) for the indicated times. Cell lysates (500 μg) were immunoprecipitated with Dynabeads which can bind with TAP tag and then immunoblotted with the indicated antibodies. (b), HEK293T cells were co-transfected with IRF3-Flag, Brd3-Myc and p300 expressing plasmid for 48 hours and then infected with VSV (MOI = 0.01) for 8 hours. Cell lysates (500 μg) were immunoprecipitated with anti-Flag antibody and then immunoblotted with the indicated antibodies. Similar results were obtained in three independent experiments.

Back to article page