Figure 5: Brd3 associates with p300 and enhances the interaction between IRF3 and p300. | Scientific Reports

Figure 5: Brd3 associates with p300 and enhances the interaction between IRF3 and p300.

From: Bromodomain protein Brd3 promotes Ifnb1 transcription via enhancing IRF3/p300 complex formation and recruitment to Ifnb1 promoter in macrophages

Figure 5

(a), RAW264.7 cells were infected with VSV(MOI = 10) for the indicated times. Cell lysates (500 μg) were immunoprecipitated with Brd3 antibody and then immunoblotted with the indicated antibodies. (b), Brd3-ko cells and control cells were infected with VSV (MOI = 10) for 8 hours. Nuclear proteins were extracted and immunoprecipitated (250 μg) with IRF3 antibody and then immunoblotted with the indicated antibodies. (c), HEK293T cells were co-transfected with IRF3-Flag, Brd3-Myc and p300 expressing plasmid. After 48 hours, cell lysates (250 μg) were immunoprecipitated with anti-Flag antibody and then immunoblotted with the indicated antibodies. Similar results were obtained in three independent experiments.

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