Figure 6: Brd3 recruits IRF3/p300 complex to the promoter of Ifnb1 and facilitates the transcription of Ifnb1. | Scientific Reports

Figure 6: Brd3 recruits IRF3/p300 complex to the promoter of Ifnb1 and facilitates the transcription of Ifnb1.

From: Bromodomain protein Brd3 promotes Ifnb1 transcription via enhancing IRF3/p300 complex formation and recruitment to Ifnb1 promoter in macrophages

Figure 6

(a), Brd3-ko cells and control cells were infected with VSV(MOI = 10) for 8 hours or left unstimulated (Med). The recruitment of IRF3 and p300 to the Ifnb1 gene promoter was detected with a ChIP assay and analyzed by Q-PCR. (b), Brd3-ko cells and control cells were infected with VSV (MOI = 10) for 8 hours or left unstimulated (Med). The amount of acetylated histone3 and histone4 within Ifnb1 gene promoter was detected with a ChIP assay and analyzed by Q-PCR. Data are shown as means ± SD of three independent experiments. *P < 0.05(Student’s t-test).

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