Figure 1: The overview of experimental settings. | Scientific Reports

Figure 1: The overview of experimental settings.

From: Nesting of colon and ovarian cancer cells in the endothelial niche is associated with alterations in glycan and lipid metabolism

Figure 1

(A) Experimental design. Cancer cells from ovarian (SKOV3 and OVCAR3) and colon (HCT15 and HCT116) were co-cultured with GFP+ endothelial cells (E4+EC) for 6, 18, 24 and 48 hours. Cells were trypsinized, stained with APC-EpCAM and separated by sorting. The control cancer cells at time 0, which were not cultivated with E4+EC, were also sorted. The metabolite intensities were measured on Metabolon platform. Only metabolites, which were present in all cell lines, were used for further analysis. (B) PCA plot is showing strong separation reflecting cancer cell line.

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