Figure 1: Identification of miRNAs involved in decidualization.
From: Loss of miR-542-3p enhances IGFBP-1 expression in decidualizing human endometrial stromal cells
(A) Scatter plot of miRNA microarray results. Total RNA from three independent HESC cultures, decidualized or not, were labelled and hybridised separately on the Agilent miRNA microarray, and signal intensity, which corresponds to the expression level of each miRNA, was measured. The intensity was averaged and converted to a log2 value. Expression level of each miRNA is shown as a scatter plot. The level of expression in undifferentiated and decidualizing HESCs is shown on the Y-axis and X-axis, respectively. qRT-PCR analysis of miR-542-3p and IGFBP1 mRNA expression in undifferentiated and differentiating HESCs in samples used for microarray experiments. (B) The level of miR-542-3p (miR-542-3p/U6 ratio) expression in HESCs treated with 8-br-cAMP and MPA for 6 days was significantly down-regulated in comparison to untreated HESCs. (C) IGFBP1 mRNA expression, normalized to GAPDH mRNA (glyceraldehyde phosphate dehydrogenase), in HESCs treated with 8-br-cAMP and MPA for 6 days was significantly up-regulated in comparison to undifferentiated HESCs. The experiments were performed using three independent primary cell cultures. The data are shown as mean ± SEM. **P < 0.01.
