Figure 3: IGFBP1 is a direct target of miR-542-3p.
From: Loss of miR-542-3p enhances IGFBP-1 expression in decidualizing human endometrial stromal cells
(A) The predicted target sequence of miR-542-3p in the 3′ untranslated region (UTR) of IGFBP1 and the mutated sequence used in the luciferase assay are shown. (B) Relative luciferase activity of IGFBP1-wt and IGFBP1-mut reporter vectors co-transfected with miR-542-3p mimic (miR-542-3p) or negative control (NC) miRNA in decidualized HESCs. Using IGFBP1-wt vector, the relative luciferase activity was decreased by 31% upon transfection with miR-542-3p compared to NC mimic. The IGFBP1-mut reporter was not significantly inhibited by miR-542-3p mimic. Data are shown as the mean ± SEM of three independent experiments. *P < 0.05; NS, Not significantly different.
