Table 4 Formulation and proximate composition of the experimental diets (% of dry matter).

From: Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish

Ingredients

FO 1

FV 2

VO 3

Defatted white fish meal 4

15

15

15

Soybean meal

32

32

32

Casein 5

11

11

11

Wheat meal

26

26

26

Mineral premix 6

2

2

2

Vitamin premix 7

2

2

2

Attractant 8

0.3

0.3

0.3

Mould inhibitor 9

0.1

0.1

0.1

Lecithin

2.6

2.6

2.6

Fish oil

9

4.5

0

Soybean oil

0

2.5

4.5

Linseed oil

0

2.5

4.5

Total

100

100

100

Proximate composition

 Crude protein

41.67

41.74

41.71

 Crude lipid

12.85

12.70

12.76

  1. 1FO: 100% Fish oil as lipid source (control).
  2. 2FV: Vegetable oil blend (linseed oil: soya bean oil = 1:1) replacing 50% of fish oil.
  3. 3VO: 100% Vegetable oil blend as lipid source.
  4. 4Defatted fish meal: 72.1% Crude protein and 1.4% crude lipid; white fish meal were defatted with ethanol (fish meal: ethanol = 1:2, w:v) at 37 °C three times.
  5. 5Casein: 88% Crude protein and 1.3% crude lipid, Alfa Aesar, Avocado Research Chemicals Ltd, UK.
  6. 6Mineral premix (mg or g kg-1 diet): CuSO4·5H2O 10 mg; Na2SeO3 (1%) 25 mg; ZnSO4·H2O, 50 mg; CoCl2·6H2O (1%) 50 mg; MnSO4·H2O 60 mg; FeSO4·H2O 80 mg Ca (IO3)2 180 mg; MgSO4·7H2O 1200 mg; zeolite 18.35 g.
  7. 7Vitamin premix (mg or g kg−1 diet): Vitamin D 5 mg; vitamin K 10 mg vitamin B12 10 mg vitamin B6 20 mg; folic acid 20 mg; vitamin B1 25 mg; vitamin A 32 mg; vitamin B2 45 mg; pantothenic acid 60 mg; biotin 60 mg; niacin acid 200 mg; α-tocopherol 240 mg; inositol 800 mg; ascorbic acid 2000 mg; microcrystalline cellulose 16.47 g.
  8. 8Phagostimulant: Glycine: betaine = 1:3.
  9. 9Preservative: Fumarate: calcium propionate = 1:1.
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