Table 6 Primers used in real-time PCR.
From: Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish
Species | Gene | Primer | Sequence (5′-3′) |
|---|---|---|---|
Rainbow trout | SREBP-1 | RS-F | CAAGCTGCCCATCAACCGTA |
RS-R | GGCCACCAGGTCTTTAAGCTC | ||
PPAR-α1 | RP1-F | TCCCCCAGTCCATCGACGGTGACA | |
RP1-R | GAGAACTCCTCCAGCCCTGCAGCT | ||
PPAR-α2 | RP2-F | CAGTCGAGTAACTGCTCTGGCT | |
RP2-R | ACAGGCGTGAACTCCATAGTGGT | ||
FADS2 | RFADS2-F | GTCCGTGCTTTGTGTGAGAA | |
RFADS2-R | TCAGAGACCCGACAACATCA | ||
β-actin | R-β-actin-F | TCCTTCCTCGGTATGGAGTCT | |
R-β-actin-R | TTACGGATGTCCACGTCACAC | ||
Japanese seabass | SREBP-1 | JS-F | TGCTATCGGTTCTAACATGGCTAC |
JS-R | AGTGCTCAACAGTCAGATACAGTC | ||
PPAR-α1 | JP1-F | AAGACCAGCACCCCTCCTTTCGT | |
JP1-R | CCGAACTTCTGCCTCCCTGTCCT | ||
PPAR-α2 | JP2-F | TTCCAGCTGGCAGAGAGGACGC | |
JP2-R | CACCCCACAGCCGGAACCACCT | ||
FADS2 | JFADS2-F | CCGTCGCGACTGGGTGGATATG | |
JFADS2-R | CAGTCCCGGTGCTTCTCGTGG | ||
β-actin | J-β-actin-F | CAACTGGGATGACATGGAGAAG | |
J-β-actin-R | TTGGCTTTGGGGTTCAGG | ||
Large yellow croaker | SREBP-1 | YS-F | TCTCCTTGCAGTCTGAGCCAAC |
YS-R | TCAGCCCTTGGATATGAGCCT | ||
PPAR-α1 | YP-F | AAGTGCCTCTCTGTGGGAATGT | |
YP-R | TCACCTCTTTCTCCACCATCT | ||
FADS2 | YFADS2-F | TTCGCTTCCTCTGCTGCTATG | |
YFADS2-R | CCAGTCACGGTGCTTCTCG | ||
β-actin | Y-β-actin-F | CTACGAGGGTTATGCCCTGCC | |
Y-β-actin-R | TGAAGGAGTAACCGCGCTCTGT |
