Figure 5: W. cibaria WIKIM28 isolated from gatkimchi enhance the gene expression of tolerogenic DC markers and the proportion and function of Tregs. | Scientific Reports

Figure 5: W. cibaria WIKIM28 isolated from gatkimchi enhance the gene expression of tolerogenic DC markers and the proportion and function of Tregs.

From: Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice

Figure 5

(a,b) The expression levels of ICOS-L and PD-L1 in BMDCs were measured by real-time PCR (a) and flow cytometry (b). The mean fluorescence intensity (MFI) of PD-L1 and ICOS-L on BMDCs. (c,d) After incubation of BMDCs with WIKIM28, activated DCs were co-cultured with naïve CD4+ T cells for 5 days on anti-CD3/CD28 mAbs-coated plates. The CD4+CD25+Foxp3+ Treg proportion was then analyzed by flow cytometry (c). IL-10, IFN-γ, IL-4, and TNF-α production in the supernatant was quantitated by flow cytometry using a Cytometric Bead Array kit (d). Error bars indicate SE. Data are representative of three independent experiments. *p < 0.05, **p < 0.001, compared to the negative control group (No LAB). #p < 0.05, ##p < 0.001, compared to the LPS.

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