Figure 2: Fast scanless mapping of sparse cortical dynamics in vivo.
From: Simultaneous high-speed imaging and optogenetic inhibition in the intact mouse brain
(a) Fluorescence signals over time from 21 cortical neurons, imaged in the scanless configuration at 1 kHz in an anesthetized mouse. λ = 920 nm; power, <13 mW per spot. Depth: 140 μm. ΔF/F0 are shown in grey; black lines represent a posteriori filtered traces (τ = 15 ms, see Methods). (b) Scanning image showing the GCaMP6f-expressing neurons imaged in (a). The red crosses indicate the positions of the spots used for scanless imaging. Each cell is identified with a number in (b). Cell number 0 was not plotted in (a) and used only as spatial reference. (b1) Fluorescence signals recorded with the camera during scanless multipoint illumination of the neurons indicated in (b). Four pseudocolor scales are shown corresponding to the four camera detector quadrants (see Methods for details). (c,d) Instantaneous network correlation as a function of time (c) and pair-wise correlation (d) for the experiment displayed in (a). (e1–e3) Raster plots of the calcium transient onsets (see Methods) for the three recordings shown in (a).
