Figure 4: FLIM studies of the aggregation of N47A-SH3-DA.
From: Two-Step Amyloid Aggregation: Sequential Lag Phase Intermediates
(a) A488 FLIM images of N47A-SH3-DA incubated at 32 μM. Aliquots were collected at different incubation times and diluted to 280 pM for imaging. The white scale bars represent 2.4 μm. (b) Average τA488 values vs. incubation time from the FLIM images of aliquots of the N47A-SH3-DA mixture at 24 (red), 28 (black), and 32 μM (blue). The error bars represent the s.d. of the τA488 values obtained from at least 10 different FLIM images. The lines indicate the fits to a single-exponential function.
