Figure 7: Alterations in adenosine A1 and A2a receptor expression in Ada−/− mice. | Scientific Reports

Figure 7: Alterations in adenosine A1 and A2a receptor expression in Ada−/− mice.

From: Alterations in the brain adenosine metabolism cause behavioral and neurological impairment in ADA-deficient mice and patients

Figure 7

(a) TaqMan gene expression analyses for adenosine receptor A1. Olfactory bulbs, thalamus, cortex, cerebellum and hippocampus ex vivo; ΔCt normalized for GADPH endogenous control (average of 6 experiments + SEM). (b) TaqMan gene expression analyses for adenosine receptor A2a. Olfactory bulbs, thalamus, cortex, cerebellum and hippocampus ex vivo; ΔCt normalized for GADPH endogenous control (average of 6 experiments + SEM). (c) Representative cropped Western Blot analysis for A1 (36 kDa), A2a (45 kDa) and ERK1/2 (42/44 kDa) as housekeeping control on brain fractions from a Ada+/+, Ada−/− and Ada−/− mouse treated with ERT. Total protein preparation, cell debris and nuclei (P1) and postnuclear supernatant (S1), synaptosomal fraction (P2) and supernatant (S2), microsomal pellet (P3) and post-microsomal soluble protein fraction (S3).

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