Figure 1: Expression of CXCR4 and SDF-1 and their role in promoting migration of hDPSCs. | Scientific Reports

Figure 1: Expression of CXCR4 and SDF-1 and their role in promoting migration of hDPSCs.

From: SDF-1/CXCR4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3β/β-catenin pathways

Figure 1

(A) RT-PCR analysis of CXCR4 mRNA in hDPSCs. (B) CXCR4 was identified by Western blot in hDPSCs. HeLa cells (A and B) were taken as positive control. (C) Immunofluorescence analysis of CXCR4 expression in hDPSCs. Cells not incubated with primary antibodies were taken as negative control. (D) The hDPSCs were cultured in medium with the indicated dose of SDF-1 for 48 hours and subjected to Western blot using CXCR4 antibody. (E) The hDPSCs were used in Transwell assays with the indicated dose of SDF-1 in the lower well. Cells that migrated to the lower side of the well were fixed and stained for 12 hours. Representative images are shown. The quantitative results (D and E) are the means ± SD of three independent experiments. Comparison with the control group (0 ng/ml): *p < 0.05; #p < 0.01. In (C), scale bar = 100 μm; in (E), scale bar = 200 μm.

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