Figure 2: SDF-1 mediated concentration-dependent and time-dependent effects on FAK, PI3K, Akt, GSK3β and β-catenin protein expression. | Scientific Reports

Figure 2: SDF-1 mediated concentration-dependent and time-dependent effects on FAK, PI3K, Akt, GSK3β and β-catenin protein expression.

From: SDF-1/CXCR4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3β/β-catenin pathways

Figure 2

(A) The hDPSCs cultured in medium with the indicated dose of SDF-1 for 2 hours were subjected to Western blots. (B) The hDPSCs cultured in medium with SDF-1 (50 ng/ml) for the indicated times were detected by Western blot. Representative images and quantitative results (A and B) are shown. The membrane expression of p-FAK (C) and the nucleus translocation of β-catenin (D) were obtained after 50 ng/ml SDF-1 stimulation for 6 hours. Cells without SDF-1 stimulation were taken as control. The quantitative results (A and B) are the means ± SD of three independent experiments. The white arrows (C) indicate p-FAK at the leading edge. Comparison with the control group: *p < 0.05; #p < 0.01. In (C) and (D), scale bars = 50 μm.

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