Figure 3: AMD3100 suppressed SDF-1/CXCR4 signaling and hDPSCs migration.

(A) After preincubation with AMD3100 (100 ng/ml) for 1 hour, hDPSCs were used in Transwell assays with AMD3100 in the upper chamber and SDF-1 in the lower chamber and allowed to migrate for 12 hours. (B) After preincubation with AMD3100 for 1 hour and culture in medium with the indicated drug concentrations (100 ng/ml AMD3100, 50 ng/ml SDF-1) for 2 hours, cells were subjected to Western blots. The quantitative results (A and B) are the means ± SD of three independent experiments. NS, not significant; *p < 0.05. In (A), scale bar = 200 μm.