Figure 4: Downregulation of CXCR4 by siRNA transfection decreased the migration of hDPSCs and SDF-1/CXCR4 signaling. | Scientific Reports

Figure 4: Downregulation of CXCR4 by siRNA transfection decreased the migration of hDPSCs and SDF-1/CXCR4 signaling.

From: SDF-1/CXCR4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3β/β-catenin pathways

Figure 4

(A) After transfection with siCXCR4 for 48 hours, hDPSCs were subjected to Western blot and CXCR4 was detected with the indicated antibody. (B) Cells transfected with siCXCR4 were used in Transwell assays with the indicated medium (50 ng/ml SDF-1) in the lower chamber. Cells that migrated to the lower side of the well were fixed and stained for 12 hours. Representative images are shown. (C) After transfection with siCXCR4 for 48 hours, cells were cultured in the indicated medium (50 ng/ml SDF-1) for 2 hours and subjected to Western blots. The quantitative results (A,B and C) are the means ± SD of three independent experiments. NS, not significant; *p < 0.05; #p < 0.01. In (B), scale bar = 200 μm.

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