Figure 5: PI3K inhibition by LY294002 played a negative role during the migration of hDPSCs. | Scientific Reports

Figure 5: PI3K inhibition by LY294002 played a negative role during the migration of hDPSCs.

From: SDF-1/CXCR4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3β/β-catenin pathways

Figure 5

(A) After preincubation with LY294002 (20 μM) for 1 hour, hDPSCs were used in Transwell assays with LY294002 in the upper chamber and SDF-1 in the lower chamber and allowed to migrate for 12 hours. (B) After preincubation with LY294002 for 1 hour and culture in medium with the indicated drug concentrations (20 μM LY294002, 50 ng/ml SDF-1) for 2 hours, cells were subjected to Western blots. The quantitative results (A and B) are the means ± SD of three independent experiments. NS, not significant; *p < 0.05; #p < 0.01. In (A), scale bar = 200 μm.

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