Figure 3: Molecular detection of transgenic cassava stability.

(A) PCR products of MeCu/ZnSOD and MeCAT1 as presented on 1% agarose gel. Lane M: DNA standard ladder (DL-2000); lanes 1, 3, 5, and 7: PCR products were amplified using genomic DNA of wild type (WT), SC2, SC4, and SC11 as templates, respectively, and primers were specific for MeCu/ZnSOD; lanes 2, 4, 6, and 8: PCR products were amplified using genomic DNA of WT, SC2, SC4, and SC11 as templates, respectively, and primers were specific for MeCAT1. (B) qRT-PCR analysis of transcript levels of MeCu/ZnSOD and MeCAT1 in both transgenic cassava lines and WT. The relative transcript levels of MeCu/ZnSOD and MeCAT1 in the WT were set as 1.0, and the transcript levels were presented as the ratios of MeCu/ZnSOD and MeCAT1 in transgenic cassava lines, WT and C1115.