Table 3 Primers used for PCR and qRT-PCR detection of transgenic cassava lines.
Application | Primer name | Sequence (5′—3′) | Tm (°C) | Product length (bp) |
|---|---|---|---|---|
PCR | MeCu/ZnSOD-F | ATGGCCCTCCATTATTTACACT | 57.5 | 759 |
MeCu/ZnSOD-R | AACAACGACTGCCCTTCCTACAAT | 62.0 | ||
MeCAT1-F | TGTGAAGATAGTGGAAAAGGAAGG | 59.5 | 823 | |
MeCAT1-R | CTCAGGATGGTGTGATAAGAAGTC | 58.0 | ||
qRT-PCR | MeCu/ZnSOD(Q)-F | ATGTTCATGCCCTTGGAGAC | 58.3 | 114 |
MeCu/ZnSOD(Q)-R | GATCACCAGCATGACGAATG | 58.4 | ||
MeCAT1(Q)-F | TGGGAAACAACTTCCCTGTC | 59.4 | 172 | |
MeCAT1(Q)-R | ACATCATCGAAGAACCAGGC | 58.5 | ||
Meactin(Q)-F | TGATGAGTCTGGTCCATCCA | 59.8 | 108 | |
Meactin(Q)-R | CCTCCTACGACCCAATCTCA | 60.2 |
