Figure 4: Comparison ClyF activity with other lysins.
(A) Comparison the lytic rate (−mOD600nm/min) of ClyF against S. aureus N315 with that of four other chimeolysins discovered on the screening plates. (B) The changes of OD600nm of S. aureus N315 exposed to an equal molar amount (0.35 μM) of ClyF and PlySs2. (C) Lytic rates of ClyF, PlySs2 and Pc (0.35 μM each) against several S. aureus clinical isolates. (D) Log killing abilities of ClyF and Pc against S. aureus N315 in milk and serum. S. aureus N315 resuspended in pasteurized milk and mouse serum were treated with different concentrations (0–1.4 μM) of ClyF or Pc at 37 °C for 1 h, respectively, the residual viable cell number was calculated by plating serial dilutions onto Baird-Parker agar plates. Each assay was repeated for at least three times. Mean values are plotted, and error bars represent 1× standard deviation.
