Figure 6: Efficacy of ClyF in a systemic mouse model of MRSA bacteremia.
(A) Dose-dependent rescue curves of ClyF. Mice were intraperitoneally injected with 6 × 107 CFU of MRSA WHS11081. Three hours post-injection, animals are divided into four groups randomly. Three groups (6 each) received 25, 37.5 and 50 mg/kg ClyF intraperitoneally, respectively, and the fourth group (n = 5) was injected with PBS buffer only (Blank line). Another group (n = 3) without MRSA infection was given 100 mg/kg ClyF only (Red line). The survival rates of all the groups were recorded over 7 days. Survival data of ClyF treated groups were analyzed by one-way ANOVA in comparison with that of the PBS treated control. * denotes p < 0.05. (B) Titers of anti-ClyF antibody in mice serum. The nonimmunized mouse serum was used as control. Error bars represent standard deviation. (C) Effect of ClyF-immunized serum on the lytic activity of ClyF in vitro. The nonimmunized mouse serum was used as negative control.
