Figure 7: Efficacy of ClyF in a mouse model of burn wound infection.

(A) Log killing efficacy of ClyF against S. aureus biofilms formed in burn wounds. S. aureus WHS11081 was inoculated onto the wounded dorsum skin of each mouse for 24 h to allow biofilm formation. After applied topically with ClyF for 24 h, the bioburden in each skin sample was homogenized and calculated by plating onto Baird-Parker agar plates. Untreated and PBS treated groups were used as controls. Data was analyzed by one-way ANOVA, each cycle represents an individual mouse, bars indicate mean, and * denotes p < 0.05. (B) H&E and Gram staining analysis of S. aureus biofilms in burn wounds. Tissue sections were imaged at x400 magnification by an automatic digital slide scanner. Bar: 100 μm. (C) SEM analysis of the S. aureus biofilms in burn wounds. Images were taken at x20,000 magnification under the same instrument conditions. ClyF* represents the group treated with 0.1 mg ClyF twice in a 6 h interval. Bar: 1 μm.