Table 1 Transplantation results for WC/R26-LacZ testicular cells with mECM.

From: Mammary extracellular matrix directs differentiation of testicular and embryonic stem cells to form functional mammary glands in vivo

Cell Source

Treatment

Xgal+ mammary outgrowth/total inoculations

Xgal+ second generation outgrowths inoculations

7.5 × 104 Testicular Cells

Nulliparous Mouse mECM

16/62

ND

7.5 × 104 Testicular Cells

Omental Fat ECM

0/14

N/A

7.5 × 104 Testicular Cells

Lung ECM

0/19

N/A

7.5 × 104 Testicular Cells

DMEM

0/15

N/A

None

Nulliparous Mouse mECM

0/10

N/A

5 × 104 Testicular Cells

Nulliparous Rat mECM

4/18

5/8

5 × 104 Testicular Cells

Involuting Rat mECM

6/19

ND

5 × 104 Testicular Cells

DMEM

0/20

N/A

  1. Cell/ECM mixtures were inoculated into the epithelium divested (cleared) mammary fat-pads of 3–4 week old female nude mice. Mice were impregnated and allowed to involute for 3 weeks to activate the WC/R26-LacZ reporter. Second generation outgrowths were generated by transplanting fragments from Xgal+ primary outgrowths into cleared mammary fat-pads of 3–4 week female nude old mice and allowed to grow for 8 weeks. ND = not determined; N/A = not applicable.
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