Figure 2: mAb DTE41 preferentially recognises microtubule enriched by the glutamylated Δ2 form in cultured cells. | Scientific Reports

Figure 2: mAb DTE41 preferentially recognises microtubule enriched by the glutamylated Δ2 form in cultured cells.

From: Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer’s disease

Figure 2

(a) Representative western blot analyses with 3 different antibodies in HEK293T cells. Original full-length blot images were provided in Supplementary Figure 6b. (b) Quantitative analyses of western blotting as shown in panel a. Results are shown as the mean of four independent experiments. Error bars represent S.E.M. (c) Immunocytochemical analyses of mAb DTE41 in COS7 cells. mAb DTE41 was reactive only to cells expressing both TTLL5 and CCP1 (arrowhead). Scale bar, 20 μm. Green, EGFP-TTLL5; red, DTE41; blue, CCP1-flag. (d) Quantification of mAb DTE41-positive cells in panel c. (e,f) Co-labelling of modified microtubule with mAb DTE41, mAb GT335, and anti-Δ2-tubulin pAb. COS7 cells or IMCD3 cells co-expressing TTLL5 and CCP1 were labelled with mAb DTE41 and mAb DM1A, mAb GT335, or anti-Δ2-tubulin pAb. Red, DTE41; green, DM1A (upper), Δ2-tubulin (middle), or GT335 (bottom); blue, DAPI. Panel f shows magnified images in a box of dashed line in panel e. Scale bar, 10 μm.

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