Figure 3: Evaluation of exopolysaccharide abundance in cultures of clinical isolates as compared to the reference strain PAO1 as a function of temocillin MICs. | Scientific Reports

Figure 3: Evaluation of exopolysaccharide abundance in cultures of clinical isolates as compared to the reference strain PAO1 as a function of temocillin MICs.

From: Mechanisms of intrinsic resistance and acquired susceptibility of Pseudomonas aeruginosa isolated from cystic fibrosis patients to temocillin, a revived antibiotic

Figure 3

Calcofluor white (CFW) fluorescence was measured for 73 clinical isolates, including those for which mexA and mexB were sequenced (Table 1). Data expressed as a ratio to the value measured for the non-mucoid reference strain PAO1 (highlighted by the dotted line), the MIC of which is 512 mg/L). The red horizontal lines show the mean of values for 7–8 isolates for each MIC (3 only for 32 mg/L [no more isolates with this MIC value in the whole collection]). Statistical analysis: figures above each bar represents the p value (multiple t-test) versus PAO1. Node splitting value for significant increase in CFW fluorescence signal vs. PAO1: MIC = 4 mg/L or higher LogWorth statistic: 1.6343 [p 0.02].

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