Table 3 Influence of porins on antibiotic activity in P. aeruginosa PA14 and its single disruptants for the oprD, opdK, or opdF genes.

From: Mechanisms of intrinsic resistance and acquired susceptibility of Pseudomonas aeruginosa isolated from cystic fibrosis patients to temocillin, a revived antibiotic

Strain

Antibiotic MIC (mg/L) a

TMO

CAR

MEM

IPM

control

+VNL

+L-Arg

control

+VNL

+L-Arg

control

+VNL

+L-Arg

control

+VNL

+L-Arg

PA14

256

384

256

48

64

48

0.37

0.37

1

1

1

2

PA14::oprD

256

384

256

48

64

48

3

3

3

4

4

4

PA14::opdKb

384

384

384

64

64

64

0.37

0.37

1

1

1

2

PA14::opdFb

384

786

384

64

128

64

0.37

0.37

1

1

1

2

  1. aMICs (mg/L) of temocillin (TMO), carbenicillin (CAR), imipenem (IPM), and meropenem (MEM) were determined in the presence of 2 mM vanillate (VNL) or 10 mM L-Arg as competitor substrates for OpdK and OprD, respectively. Values in bold highlight decreased activity vs. the corresponding control (no porin substrate added); values in italics highlight decreased activity vs the wild-type strain PA14.
  2. bTwo different clones transformed with pG-OpdK or pG-OpdF tested.
Back to article page