Figure 4: High-fat diet-induced obesity induces TRIP-Br2 expression in brown fat. | Scientific Reports

Figure 4: High-fat diet-induced obesity induces TRIP-Br2 expression in brown fat.

From: Transcription regulator TRIP-Br2 mediates ER stress-induced brown adipocytes dysfunction

Figure 4

qPCR analysis of TRIP-Br2 (a), ER stress markers BiP (b), or CHOP (c) gene expression in brown adipose tissue harvested from mice after 12 wk of CD, HFD, or HFD with TUDCA (i.p. 250 mg/kg at 8 am and 8 pm twice each day for 15 days) (n = 5); (d) Western blot analysis of TRIP-Br2, BiP, CHOP, or β-actin (loading control) in brown adipose tissue harvested from mice after 12 wk of CD, HFD, or HFD with TUDCA (i.p. 250 mg/kg at 8 am and 8 pm twice each day for 15 days). Uncut blots are included in the Supplementary information. qPCR analysis of TRIP-Br2 (e,h), ER stress markers BiP (f,i), or CHOP (g,j) gene expression in brown adipose tissue harvested from mice after 4 or 8 wk of CD or HFD (n = 5); All qPCR data are normalized to TBP and presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 (vs Ctrl or CD); #p < 0.01; ##p < 0.01 (vs HFD).

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