Figure 5: TRIP-Br2 mediates ER stress-induced downregulation of lipolysis in brown adipocytes.
From: Transcription regulator TRIP-Br2 mediates ER stress-induced brown adipocytes dysfunction

(a) Isoproterenol-stimulated lipolysis (1 μM for 2 h) in WT1 differentiated brown adipocytes treated with vehicle or tunicamycin (1 μg/ml) for 6 or 24 h (n = 3). qPCR analysis of (b) Hsl (c) Atgl gene expression in WT1 differentiated brown adipocytes treated with vehicle or tunicamycin (1 μg/ml) for 6 or 24 h (n = 3). *p < 0.05; ***p < 0.001 (vs Ctrl). (d) Protein expression of ATGL and β-actin (loading control) in WT1 differentiated adipocyte treated with vehicle or tunicamycin (1 μg/ml) for 6 or 24 h. Uncut blots are included in the Supplementary information. (e) Isoproterenol-stimulated lipolysis (1 μM for 2 h) in WT and TRIP-Br2 KO brown adipocyte treated with vehicle or tunicamycin (1 μg/ml) for 24 h (n = 6). (f) mRNA expression of Hsl and Atgl in WT and TRIP-Br2 KO brown adipocytes treated with vehicle or tunicamycin (1 μg/ml) for 24 h (n = 6). All qPCR data are normalized with TBP and presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 (vs WT-Ctrl); #p < 0.05; ##p < 0.01; ###p < 0.001 (vs WT-Tuni).