Figure 4: RNA interference knockdown of transcripts of putative S. exigua Cry2Aa-binding proteins in S. exigua larvae and subsequent susceptibility of larvae to Cry2Aa toxin.
From: Proteomic analysis of Cry2Aa-binding proteins and their receptor function in Spodoptera exigua
(a) Relative expression of target gene transcript levels in S. exigua neonate larvae that had been allowed to feed for 48 h on an artificial diet to which either water, EGFP dsRNA, or Se-target genes dsRNA, had been added. Relative estimates of target gene transcript levels were obtained by qRT-PCR and normalized to those of RpL10 and GAPDH. *P < 0.05 (ANOVA). (b) Corrected mortality of different treatment groups after feeding on a diet containing 2.6 μg/cm2 Cry2Aa toxin. The corrected mortality is based on 5 replicates. Bars with different letters indicate P values < 0.05 (ANOVA).
