Figure 4: Histological analysis of calcific vessels.

Representative immunostaining of OPN and CD14 (A), OPN and tartrate-resistant acid phosphatase (TRAP) (B) on blood vessels from hypertensive patients without vascular calcification (HTN-NO-VC) and with vascular calcification (n = 5 per group). CD14 represents macrophages, TRAP represents osteoclasts. DAPI (4′, 6-diamidino-2-phenylindole) was used to detect nucleus Bar: 100 μm.