Table 8 Primers sequences for qPCR.
Name | Protein name | Sequences: Forward (F) and Reverse (R) | Size (bp) | NCBI number |
|---|---|---|---|---|
Runx2 | Runt-related transcription factor 2 | F: 5′-GACAAGCACAAGTAAATCATTGAACTACAG-3′ R: 5′-GTAAGGCTGGTTGGTTAAGAATCTCTG-3′ | 196 | NM_001024630.3 |
ALP | Alkaline phosphatase | F: 5′-GGACACTGGGCATAGATTTCTCAAG-3′ R: 5′-TGCTGGGATTACAAACACTTCCTTT-3′ | 109 | NM_001632.4 |
Col1 | Collagen type I | F: 5′-GTCACCCACCGACCAAGAAACC-3′ R: 5′-AAGTCCAGGCTGTCCAGGGATG-3′ | 121 | NM_000088.3 |
Bglap | Osteocalcin | F: 5′-CCTCACACTCCTCGCCCTATT-3′ R: 5′-CCCTCCTGCTTGGACACAAA-3 | 117 | NM_199173.5 |
Spp1 | Osteopontin | F: 5′-GTACCCTGATGCTACAGACG-3′ R: 5′-TTCATAACTGTCCTTCCCAC-3′ | 139 | NM_001040058.1 |
TRAP | Tartrate-resistant acid phosphatase | F: 5′-TGACAAGAGGTTCCAGGAGAC-3′ R: 5′-GAAGTGCAGGCGGTAGAAAG-3′ | 178 | NM_001111035.1 |
CTSK | Cathepsin K | F: 5′-AACGAAGCCAGACAACAGATT-3′ R: 5′-CGAGAGATTTCATCCACCTTGTT-3′ | 170 | NM_000396.3 |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | F: 5′-TGCACCACCAACTGCTTAGC-3′ R: 5′-GGCATGGACTGTGGTCATGAG-3′ | 178 | NM_002046.5 |
