Figure 1: Screening S. cerevisiae deletants of sphingolipid biosynthetic pathway genes.

An overview of sphingolipid biosynthetic pathway is shown in Fig. S1. Ten-fold serial dilutions of cells were spotted onto synthetic complete agar plates with indicated concentration of (a) calcofluor white (CFW), or (b) congo red (CR). Plates were incubated at 30 °C for 2 days before being photographed.